Culture dependent vs culture independent methods Lab Report

Culture dependent vs culture independent methods - Lab Report Example 104). Some of the techniques that can be applied include, but not limited to performing rDNA PCR amplification on clinical specimens regarded sterile, such as blood. It is however advisable that this technique should not be employed with specimens originating from nonsterile sites such as faeces (Litton, 2010 p. 56). Collection of specimens such as conventional assays needs aseptic precautions. Litton (2010, p.57) claims that in order to curb contamination DNA brought by specimen collection vials, ensuring that the environment where work goes on is well organised also helps with this. Contamination linked to personnel working in the laboratory can be avoided by wearing cloves made of gloves or latex plus white coats. As for those contaminations resulting from consumable reagents and plastic wares, prior screening of each and every reagent before use in diagnostic assays (Litton, 2010 p. 56). 3. Find at least one peer-reviewed scientific research article regarding the bacteria that normally reside in the human mouth and provide references. Describe the groups of bacteria these studies identified to be present in the human mouth. Some of the bacteria that reside in the mouth include but not limited to, staphylococcus with the most common ones being S.epidermidis and S.aureus. They are oval in shape and posses a thick cell wall, named gram-positive. They cause infections in human population when presented with optimal conditions (â€Å"New bacterial species found in human  mouth† 2008, p.26). Bacteria from the genus streptococcus forms the largest number of all the organisms found in the mouth. Some of the species here include, but not restricted to, S. mutans, S. mitis, S. salivarius, S. pneumoniae and S. Pyogenes. They are also oval in shape like the staphylococcus. S.mutans is also responsible for cavity formation in teeth by converting sucrose sugar into lactic acid which

Literature Review for Concept Analysis Dissertation

Literature Review for Concept Analysis - Dissertation Example This is the premise in which exercise became an inevitable element for sustaining a healthy life. The changes that happened in the society were also reflected in the psychological and moral realm of lives as well. Thoughts about rights and power also emerged out of leisure and the question that who are the owners of leisure, and who commands it, arose. Though Oxford Dictionaries Online has offered several definitions for the term, ‘exercise’, I will explore the following two definitions in this concept analysis, a) â€Å"activity requiring physical effort, carried out especially to sustain or improve health and fitness,† †¦[and b)]†¦ â€Å"the use or application of a faculty, right, or process† (Oxford Dictionaries Online, 2010). The first definition stresses the meaning of exercise as a physical activity while, the second one views exercise as the fulfillment of a duty and as the claiming of a right. Taking these two definitions are taken as the yardsticks to understand the importance of education, this analysis envisages to find out how exercise attains a valuable place in the three areas of knowledge, namely, theology, psychology, and nursing. Exercise in theology In a democratic society, an individual has several rights and powers, the exercise of which is supposed to be the foundation of the democratic system itself. Whenever the exercise of any such right or power is denied, the foundations of democracy feel threatened. And this is why the people of many nations around the globe had to fight prolonged battles to be able to exercise these powers and rights. For example, in America, the people felt that â€Å"the right to vote was denied for so long to so many that we cannot afford to ignore any impediment to its exercise† (Grey, 2005). In the theological realm also, there are a set of such rights and powers, which follow the democratic rights but also give rise to many contentious issues as well. For example, Pos t (1995) has observed that â€Å"free exercise is justifiably overridden to promote public health- for example, medical treatment is required to minimize the spread of contagious disease or to benefit minors, even if religious belief is offended† (p.22). Though Post (1995) has reminded, â€Å"the free exercise clause of the First Amendment is central to American public life,† from a nurse’s point of view, situations may arise when a patient wants to exercise his/her right to refuse treatment and the nurse and the system in which he/she works are compelled to use authority and power to prevent that (p. 22). This is particularly important because, just as any other freedom, the freedom to exercise one’s religion also has limits and can be overridden if public health is at risk of being jeopardized (Post, 1995). Taking into consideration these two opposite yet justifiable positions, nurses have to be aware that a patient has the right to refuse or seek treat ment based on their religion and when the right to this free exercise is dishonored, it should be examined and justified. Post (1995) has discussed a legal case in which religion, law, and medicine clash. The article is based on the true story of Baby K, a baby born with anencephaly. The mother, who believes in the sanctity-of-life principle, wants Baby K kept on a ventilator; because of her faith, she believes that a person’